Pluronic Micelles Nano-Carrier Synthesis

Figure 1: Illustration of pluronic F127 micelle presenting the hydrophobic body, blue, and hydrophilic tails, red. Note polymer ratio of hydrophilic to hydrophobic is incorrect where hydrophilic should be 70% to 30% hydrophobic.

The project modified Pluronic F127 (Flakes – ~12600 g/mol – 70% hydrophilic) a.k.a P407 (Poloxamer)1, figure 1, with an amine terminal, NH2, which formed an amide bond with CH3 terminal Rhodamine B Isocyanate2. The project used a schlenk line for the controlled vacuum for amination and nitrogen atmosphere for Rhodamine functionalisation. Dialysis was carried out removing excess Rhodamine dye from the reaction. The final solution was freeze-dried before use. Dynamic light scattering measurements of the nano-micelles presented a hydrodynamic size between 20-24nm. The project supported the work published by my previous PhD supervisor’s work, Dr. Andrew Collins3.

The project was part of my PhD at the University of Bristol4.

References

  1. Merck. (2021) Pluronic F-127 powder. [Accessed 06/10/2021] https://www.sigmaaldrich.com/GB/en/product/sigma/p2443
  2. Merck. (2021) Rhodamine B Isocyanate. [Accessed 06/10/2021] https://www.sigmaaldrich.com/GB/en/substance/rhodaminebisothiocyanate5360836877697
  3. Singleton, W. G., Collins, A. M., Bienemann, A. S., Killick-Cole, C. L., Haynes, H. R., Asby, D. J., Butts, C. P., Wyatt, M. J., Barua, N. U., & Gill, S. S. (2017). Convection enhanced delivery of panobinostat (LBH589)-loaded pluronic nano-micelles prolongs survival in the F98 rat glioma model. International Journal of Nanomedicine, 12, 1385–1399. https://doi.org/10.2147/IJN.S125300
  4. Hockley, M. (2019). Screening Nanoparticle Dynamics on Modular Tumours-on-a-chip devices [University of Bristol]. https://research-information.bris.ac.uk/en/studentTheses/screening-nanoparticle-dynamics-on-modular-tumours-on-a-chip-devi
0 Comments

Leave Your Reply

Your email address will not be published.


*